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KMID : 1011220150020020029
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Clinical & Experimental Thrombosis and Hemostasis
2015 Volume.2 No. 2 p.29 ~ p.32
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Baseline Characteristics of Derivative Peaks of Activated Partial Thromboplastin Time Coagulometric Turbidometry Curve
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Song Jae-Woo
Yoon Ji-Houn
Jo Yu-Na
Park Ro-Jin
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Abstract
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Purpose: Turbidimetric detection of clot in plasma is a major assay principle adopted by many coagulometersused in clinical laboratories. We derived local maxima of 1st and 2nd derivatives of turbidimetriccurves of activated partial thromboplastin time as introduced in other studies as peak 1 and peak 2respectively.
Methods: Peak 1 and peak 2 values were derived from APTT data stored in the operating computer. TheAPTT turbidime try data were converted into csv file format and analyzed using a spreadsheet program.Each series of first or second derivative values were scanned for local maxima, which were then chosenas peak 1 and 2 values, respectively.
Results: The coefficient of variations of peak 1 and 2 of within-run repeats were 3.8% and 8.3% for normalAPTT plasma and 4.8% and 14.8% for prolonged APTT plasma. Peak 1 inversely correlated with APTTbut the relation was not strong (r: -0.5324; 95% confidence interval: -0.7143 to -0.2832) whereas peak 2showed stronger inverse correlation (r: -0.8257; 95% CI: -0.9009 to -0.7024). The reference intervals werederived from APTT data from 115 apparently healthy individuals. There was no significant difference indistribution of peak 1 or 2 values between both sexes and the reference intervals were determined to 150.0 to 290.7 TU/sec for peak 1 and 488.4 to 1,026.3 TU/sec2 for peak 2.
Conclusion: Our study is a starting point that will aid in the study of hemostatic meaning of the new coagulometerparame ters peak 1 and 2 and on the evaluation of their clinical usefulness.
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KEYWORD
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Activated partial thromboplastin time, Turbidimetry, Clot, Derivative, Peak 1, Peak 2
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